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Pregnancy proteins - molecular biological and biochemical analysis
Muravská, Alexandra ; Kalousová, Marta (advisor) ; Černá, Marie (referee) ; Průša, Richard (referee)
The aim of this thesis was to establish methods for selected PAPP-A (Pregnancy- Associated Plasma Protein A) gene polymorphisms analysis and to study genetic background of PAPP-A and biochemical background of PAPP-A and PlGF (Placental Growth Factor) in relation to risk pregnancy. Secondly, the aim was to establish method for two-dimensional (2D) electrophoresis of amniotic fluid. Methods for analysis of ten PAPP-A gene polymorphisms were established. These polymorphisms, PAPP-A and PlGF levels were studied in together 165 women in third trimester pregnancies complicated with threatening preterm labor (n=98), preeclampsia (n=35), IUGR (Intrauterine Growth Restriction) (n=34) and ICP (Intrahepatic Cholestasis of Pregnancy) (n=15). 114 healthy pregnant women served as controls. The method for 2D electrophoresis of amniotic fluid was established. Preeclamptic patients had significantly higher frequency of TT genotype of Cys327Cys (C/T) PAPP-A gene polymorphism compared to controls. Patients with ICP had increased serum levels of PAPP-A compared to controls, in patients with threatening preterm labor PAPP-A levels were rather decreased. PlGF levels did not differ from control group in patients with ICP and threatening preterm labor. Positive correlation was found between PAPP-A and PlGF in group of...
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Pregnancy proteins - molecular biological and biochemical analysis
Muravská, Alexandra ; Kalousová, Marta (advisor) ; Černá, Marie (referee) ; Průša, Richard (referee)
The aim of this thesis was to establish methods for selected PAPP-A (Pregnancy- Associated Plasma Protein A) gene polymorphisms analysis and to study genetic background of PAPP-A and biochemical background of PAPP-A and PlGF (Placental Growth Factor) in relation to risk pregnancy. Secondly, the aim was to establish method for two-dimensional (2D) electrophoresis of amniotic fluid. Methods for analysis of ten PAPP-A gene polymorphisms were established. These polymorphisms, PAPP-A and PlGF levels were studied in together 165 women in third trimester pregnancies complicated with threatening preterm labor (n=98), preeclampsia (n=35), IUGR (Intrauterine Growth Restriction) (n=34) and ICP (Intrahepatic Cholestasis of Pregnancy) (n=15). 114 healthy pregnant women served as controls. The method for 2D electrophoresis of amniotic fluid was established. Preeclamptic patients had significantly higher frequency of TT genotype of Cys327Cys (C/T) PAPP-A gene polymorphism compared to controls. Patients with ICP had increased serum levels of PAPP-A compared to controls, in patients with threatening preterm labor PAPP-A levels were rather decreased. PlGF levels did not differ from control group in patients with ICP and threatening preterm labor. Positive correlation was found between PAPP-A and PlGF in group of...
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Informedness gravidity woman in connection with inspection of amniotic fluid.
NOVÁKOVÁ BOUŠOVÁ, Hana
The thesis should map the awareness of women in connection to the carried out investigation of amniotic fluid. On the basis of aims which should map the problems of amniotic fluid extraction four hypotheses were stated. They dealt with these questions: if pregnant women have information on amniotic fluid investigation, if pregnant women use the possibility to go for amniotic fluid investigation, if pregnant women are educated on the way of amniotic fluid investigation and if pregnant women know about the risks of the carried out amniocentesis. All hypotheses were proved and aims were fulfilled. It is possible to state two hypotheses on the basis of qualitative research. The first: pregnant women mention fear as a problem of amniocentesis. The second: pregnant women mention pain as a problem of amniocentesis. The combination of qualitative and quantitative research was used for the data collection.For the quantitative research the technique of a questionnaire was used. The questions were closed or semi-open, when informants crossed the answers or they could fill their own variation of an answer. There were 31 questions; first of these were identification questions and the other were related to the topic of the thesis itself. Expectant mothers who come to amniocentesis have enough information concerning amniotic fluid extraction but there were also some who did not have enough information. The lack of information in these mothers can be attributed to the lack of interest in amniotic fluid extraction, or inattention during education led by medical personnel. That is why the feedback during questioning is necessary. I propose the creation of leaflets and brochures which would contain everything connected to the problem of amniotic fluid extraction; the most frequent questions would be answered there. These leaflets or brochures should be available in all consulting rooms of gynaecologists and in genetic centres.
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